In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism

Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Resultsshowed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers. The dendrograms based on un-weighted pair group method arithmetic average (UPGMA) analysis of the 10, 15, 20 and the pooled (10, 15 and 20) bases RAPD data were consistent in the clustering varieties, grouping them in two main clusters

New Modulation Technique to Mitigate Common Mode Voltage Effects in Star-Connected Five-Phase AC Drives

Star-connected multiphase AC drives are being considered for electromovility applications such as electromechanical actuators (EMA), where high power density and fault tolerance is demanded. As for three-phase systems, common-mode voltage (CMV) is an issue for multiphase drives. CMV leads to shaft voltages between rotor and stator windings, generating bearing currents which accelerate bearing degradation and produce high electromagnetic interferences (EMI). CMV effects can be mitigated by using appropriate modulation techniques. Thus, this work proposes a new Hybrid PWM algorithm that effectively reduces CMV in five-phase AC electric drives, improving their reliability. All the mathematical background required to understand the proposal, i.e., vector transformations, vector sequences and calculation of analytical expressions for duty cycle determination are detailed. Additionally, practical details that simplify the implementation of the proposal in an FPGA are also included. This technique, HAZSL5M5-PWM, extends the linear range of the AZSL5M5-PWM modulation, providing a full linear range. Simulation results obtained in an accurate multiphase EMA model are provided, showing the validity of the proposed modulation approach.This work has been supported in part by the Government of the Basque Country within the fund for research groups of the Basque University system IT978-16 and in part by the Government of the Basque Country within the research program ELKARTEK as the project ENSOL (KK-2018/00040)

Suppression of the intrinsic stochastic pinning of domain walls in magnetic nanostripes

Nanofabrication has allowed the development of new concepts such as magnetic logic and race-track memory, both of which are based on the displacement of magnetic domain walls on magnetic nanostripes. One of the issues that has to be solved before devices can meet the market demands is the stochastic behaviour of the domain wall movement in magnetic nanostripes. Here we show that the stochastic nature of the domain wall motion in permalloy nanostripes can be suppressed at very low fields (0.6-2.7 Oe). We also find different field regimes for this stochastic motion that match well with the domain wall propagation modes. The highest pinning probability is found around the precessional mode and, interestingly, it does not depend on the external field in this regime. These results constitute an experimental evidence of the intrinsic nature of the stochastic pinning of domain walls in soft magnetic nanostripe